Poster Presentation 2014 International Biophysics Congress

Double-strand DNA-templated copper nanoparticles as electrochemical probes for glutathione detection (#367)

Ya Cao 1 , Xiaoli Zhu 1
  1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, China

Glutathione (GSH), one of the most abundant nonprotein biothiols in mammalian cells, plays an essential role in cellular defense against toxins and reactive oxygen species. Herein, a novel electrochemical strategy for the detection of GSH is presented. In this approach, copper nanoparticles synthesized by a double-strand DNA template (dsDNA-CuNPs) serve as efficient electrochemical probes. The strong interaction between GSH and cooper ions will hold back the formation of dsDNA-CuNPs, leading to a decreased electrochemical signal, which is related to the concentration of the target tripeptide. The sensing method is cost-effective and simple in design without requirement for complicated label or signal amplification. Under optimized conditions, the proposed method displays excellent analytical performance (e.g., a detection limit of 0.42 pM), and shows favorable usability in serum samples, providing a powerful tool for GSH detection. To the author’s knowledge, this method is the first example of dsDNA-CuNPs applied in electrochemical analysis, which may open a new avenue for electrochemical biosensor development.