Poster Presentation 2014 International Biophysics Congress

Optical and color based probes for gene-sensing (#667)

Xiaoming Zhou , Da Xing
Assays using probes labeled with optical or color moieties are extremely powerful analytical tools due to their high sensitivity or simplicity. Recently, we have developed some optical and color based probes for gene-sensing. Specifically, for optical strategies, electrochemiluminescence based on tris(2,2-bipyridyl) ruthenium(II) (Ru(bpy)32+) with tripropylamine (TPrA) as the co-reactant has been developed to combine a microfluidic system with a paramagnetic microbead solid support; For color based strategies, nucleic acids labeled gold nanoparticle probes are applied on the bioactive paper-based platform to perform a visual test using sandwich hybridization assays. The test results can be directly visualized by the naked eye for qualitative observation or by semi-quantitative spectral detection within minutes. Combing with high efficiency gene amplification, both optical and color based probes techniques shown great potential on nucleic acids analysis, which can be applied to the fields such as medical diagnostics, environmental analysis and food safety monitoring.