The rate constant (*k*_{p}) of tension-induced pore formation in lipid membranes is one of the important measures to consider the mechanical stability of lipid membranes or biomembranes. Recently, we analyzed the time-course of the fraction of intact giant unilamellar vesicles (GUVs) among all the GUVs under constant tension σ induced by micropipette aspiration, and obtained values of *k*_{p} as a function of σ^{1} . We also developed a theory to determine *k*_{p} using the mean first passage time. The fitting of the theoretical curves of *k*_{p} vs. σ to the experimental data determined the line tension of a pre-pore^{1} . Here, we investigated the effects of the electrostatic interactions on the rate constant of tension-induced pore formation in lipid membranes. The decrease in salt concentration increased the *k*_{p} values of 40% dioleoylphosphatidylglycerol(DOPG)/60%dioleoylphosphatidylcholine(DOPC)-GUVs (e.g., at σ = 5 mN/m, 1.9×10^{-2}, 1.7×10^{-3}, and 0 s^{-1} in 0, 150, and 300 mM NaCl, respectively) in a physiological buffer. The increase in surface charge density of DOPG/DOPC-GUVs increased *k*_{p} values in 150 mM NaCl in buffer. These data indicate that *k*_{p} increases as the electrostatic interactions due to the surface charges increase. We discuss the mechanism of the effects of the electrostatic interactions on *k*_{p}.

- Langmuir, 29, 3848, 2013