Poster Presentation 2014 International Biophysics Congress

Molecular basis of the receptor binding specificity switch of the hemagglutinins from both the 1918 and 2009 pandemic influenza a viruses by a D225G substitution (#436)

Jianxun Qi 1
  1. Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

Influenza A virus uses sialic acids as cell entry receptors, and there are two main receptor forms, ␣2,6 linkage or ␣2,3 linkage to galactose, that determine virus host ranges (mammalian or avian). The receptor binding hemagglutinins (HAs) of both 1918 and 2009 pandemic H1N1 (18H1 and 09H1, respectively) influenza A viruses preferentially bind to the human ␣2,6 linkage receptor. A single D225G mutation in both H1s switches receptor binding specificity from ␣2,6 linkage binding to dual receptor binding. However, the molecular basis for this specificity switch is not fully understood. Here, we show via H1-ligand complex structures that the D225G substitution results in a loss of a salt bridge between amino acids D225 and K222, enabling the key residue Q226 to interact with the avian receptor, thereby obtaining dual receptor binding. This is further confirmed by a D225E mutant that retains human receptor binding specificity with the salt bridge intact.