To investigate the influence on the expression of oncogenes and tumor suppressor genes in NIH/3T3 cells induced by 1800 MHz electromagnetic radiation (EMR).
Methods The NIH/3T3 cells were intermittently (5 min on /10 min off) exposed to 1800 MHz EMR at a specific absorption rate (SAR) of 2 W/kg in simulating GSM Talk mode. The cells were exposed to the radiation 5h/d for 60 days, during which period subcultured every 3-4 days. After exposure, a CCK-8 Kit was used to detect the viability of cells, apoptosis and cell cycle were detected by flow cytometry. Further, cells were amplified and RNAs were extracted. Then the cDNA of each sample were synthesized and added to the qPCR Array (SABiosciences) for detecting the expression of 84 oncogenes and tumor suppressor genes.
Results Compared with the sham cells, the viability of the exposed cells were reduced, cell cycle delayed in S and G2 phases, and the radiation promoted apoptosis. The RT qPCR array showed that the expression of 12 genes were significantly different between the sham and the exposed cells. Among them, 2 genes changed more than 5 folds. They were Trp53 (19.63) and Serpinb5 (8.57), both up-regulated. The 2 genes functioned as damage repair factors or tumor suppressor genes, in accordance with the cellular effects as apoptosis and cell cycle delay.