Objective Explore the Influence of fluid shear stress on the expression level of Fractalkine in human umbilical vein endothelial cells. Methods the HUVECs were divided into( 2.62 dyne/cm2，4.58 dyne/cm2，6.54 dyne/cm2，10.47 dyne/cm2，15.71 dyne/cm2，19.64 dyne/cm2) groups according to the shear stress intensity, and exposed to them for 2 hours. the shear stress intensity ,where the maximum expression level occurred was selected and the HUVECs were divided into 9 groups (control,0.5,1,2,3,5,7,9,11h) according to the disposing time. The expression of FKN mRNA in HUVECs from each group were detected by fluorescent quantitive PCR, the FKN concentration in perfusate was measured by double antibody sandwich ELISA.Result ①Under 4.58 dyne/cm2 shear stress ,the expression of FKN mRNA and the FKN concentration in perfusate increased significantly（P＜0.05）②Exposed to 4.58 dyne/cm2 shear stress for different time, maximum FKN mRNA expression was observed at 1hour group. Compared FKN mRNA expression level in 0.5h 、1h group with control、7h、9h、11h group, the difference was statistically significant, no significant difference between the other groups. Maximum protein expression level in perfusate was observed at 2h group,compared1h、2h、3h group with control、7h、9h、11h group, the difference was statistically significant（P＜0.05）,no significant difference between the other groups（P＞0.05）.Conclusion Low shear stress (4.58 dyne / cm2 )induced the increase of endothelial cell FKN synthesis and secretion in a time-dependent manner.