The aim of this investigation is to prepare and characterize self-assembled nanoarchitectures of different classes of lipids incorporating a novel, water-insoluble β-cyclodextrin derivative (βCD-C10), which possesses multiple (n=7-8) anchored hydrophobic chains (C10). First, the ability of βCD-C10 to form mixed nanostructures with dimyristoylphosphatidylcholine (DMPC) was studied. The miscibility of the amphiphiles was examined in fully hydrated self-assembled phases by differential scanning calorimetry (DSC) and X-ray diffraction. The physico-chemical study of the DMPC/βCD-C10 systems demonstrated that the amphiphilic β-cyclodextrin derivative is partially miscible with the phospholipid. A phase separation was observed above a threshold βCD-C10 molar fraction of 4 mol% in the membranes. Fluorescence spectroscopy results evidenced the incorporation of a model drug compound in the hydrophobic environment provided by the alkyl chains of the amphiphilic βCD-C10 component in the dispersed mixed objects of nanometer sizes. The formation of a βCD-C10/DMPC mixed lamellar phase occurred at βCD-C10 molar fractions up to 4 mol%, which is favourable for the preparation of liposome-type supramolecular assemblies upon dispersion. Nano-objects of βCD-C10 and polyoxyethylene oleyl ether (Brij 98) were prepared by hydration of a lyophilized film of βCD-C10 using a micellar solution of Brij 98. Quasi-elastic light scattering (QELS) and fluorescence microscopy were employed for nanoparticle characterization.