Poster Presentation 2014 International Biophysics Congress

Incorporation of amphiphilic cyclodextrins in biomimetic lipid assemblies (#252)

Leïla Zerkoune 1 , Angelina Angelova 1 , Luc Choisnard 2 , Annabelle Gèze 2 , Denis Wouessidjewe 2 , Sylviane Lesieur 1
  1. CNRS UMR8612, Institut Galien Paris-Sud, Université Paris-Sud 11, Chatenay-Malabry, France
  2. DPM, CNRS UMR 5063, University of Grenoble Joseph Fourier, Grenoble, France

The aim of this investigation is to prepare and characterize self-assembled nanoarchitectures of different classes of lipids incorporating a novel, water-insoluble β-cyclodextrin derivative (βCD-C10), which possesses multiple (n=7-8) anchored hydrophobic chains (C10). First, the ability of βCD-C10 to form mixed nanostructures with dimyristoylphosphatidylcholine (DMPC) was studied. The miscibility of the amphiphiles was examined in fully hydrated self-assembled phases by differential scanning calorimetry (DSC) and X-ray diffraction. The physico-chemical study of the DMPC/βCD-C10 systems demonstrated that the amphiphilic β-cyclodextrin derivative is partially miscible with the phospholipid. A phase separation was observed above a threshold βCD-C10 molar fraction of 4 mol% in the membranes. Fluorescence spectroscopy results evidenced the incorporation of a model drug compound in the hydrophobic environment provided by the alkyl chains of the amphiphilic βCD-C10 component in the dispersed mixed objects of nanometer sizes. The formation of a βCD-C10/DMPC mixed lamellar phase occurred at βCD-C10 molar fractions up to 4 mol%, which is favourable for the preparation of liposome-type supramolecular assemblies upon dispersion. Nano-objects of βCD-C10 and polyoxyethylene oleyl ether (Brij 98) were prepared by hydration of a lyophilized film of βCD-C10 using a micellar solution of Brij 98. Quasi-elastic light scattering (QELS) and fluorescence microscopy were employed for nanoparticle characterization.

  1. Angelova, A., Fajolles, C., Hocquelet, C., Djedaïni-Pilard, F., S. Lesieur, S., Bonnet, V., Perly, B., Le Bas, G., Mauclaire, L. J. Colloid Interface Sci., 2008, 322, 304-314.
  2. Choisnard, L., Gèze, A., Vanhaverbeke, C., Yaméogo, J.B.G., Putaux, J.-L., Brasme, B., Jullien, L., Boullanger, S., Elfakir, C., Wouessidjewe, D. Biomacromolecules, 2011, 12, 3031–3038.
  3. Lesieur, S., Charon, D., Lesieur, P., Ringard-Lefebvre, C., Muguet, V., Duchêne D., Wouessidjewe D., Chem. Phys. Lipids, 2000, 106, 127-144.
  4. Zerkoune, L., Angelova, A., Choisnard, L., Gèze, A., Wouessidjewe, D, Lesieur, S. ICOMF14 proceedings, MATEC Web of Conferences, EDP Sciences publisher, 2013, 4, 05001.